Áreas de Trabajo
BIOCHEMICAL AND MOLECULAR BIOLOGY STUDIES ON THE MICROBIAL SYNTHESIS OF SECONDARY METABOLITES.
Secondary metabolites are compounds with a wide range of chemical structures and biological activities synthesized by a few range of microorganisms usually at the late growth phase. Although involved in different functions (signalling differentiation, mediating ecological interactions and facilitating biochemical evolution in microorganisms), these metabolites are considered as secondary since are not essential for microbial growth. The remarkable diversity of biological functions and chemical structures characteristic of these metabolites reflects the random manner in which their biosynthesis has evolved and been exploited. It represents a form of inventive evolution in which metabolic opportunities that arose by chance were exploited and incorporated to various degrees into the phenotype of the species. Therefore it has been interesting for us to elucidate, at the biochemical and molecular biology levels, how these microorganisms are able to synthesize and regulate the production of these compounds. The impact of these studies can be situated at the general microbiology level, but also from the biotechnology standpoint since the resulting knowledge can be used to design rational genetic improvement and process optimization of secondary metabolites production.
PLANT-ASSOCIATED MICROBIAL SYSTEMS AS A SOURCE OF BIOLOGICALLY ACTIVE COMPOUNDS.
Plants and microorganisms synthesize most of the secondary metabolites with biological activity. Almost 50% of the biopharmaceutical have been obtained from plants. Plan-associated microorganisms (endophytic) such as bacteria or fungi produce many of these secondary metabolites. One endophytic fungi is Taxomyces andreanae, able to produce the anticancer drug, Taxol. Other endophytics have been shown to produce compounds with antibacterial, antiviral, antifungal and antimalarial activity. We are interested in the evaluation of medicinal plants as source of endophytic microorganisms as a way to look for new biologically active compounds.
Romero-Rodríguez A, Maldonado-Carmona N, Ruiz-Villafán B, Koirala N, Rocha D, and Sánchez S.
The interplay between nitrogen carbon and phosphate utilization towards the secondary metabolites production in Streptomyces species.
Antonie Leeuwenhoek, 111(5):761-781. IF: 2.51. Doi: 10.1007/s10482-018-1073-1
Ceapa C. Vázquez-Hernández M, Rodríguez-Luna SD, Cruz-Vázquez AP, Jiménez-Suárez V, Rodríguez-Sanoja R, Alvarez-Buylla E, Sánchez S.
Genome mining of Streptomyces scabrisporus NF3 reveals symbiotic features including genes related to plant interactions.
PLos One. 13(2): e0192618. DOI 10.1371/journal.pone.0192618. IF: 2.806
Sanchez, S, Rodríguez-Sanoja, R., Ramos, A., and Demain, A.L.
Our microbes not only produce antibiotics, they also overproduce amino acids.
J. Antibiot. 71-26-36 doi:10.1038/ja.2017.142- IF: 2.23
Rocha, D., Ruiz-Villafán, B., Manzo, M., Rodríguez-Sanoja, R., and Sánchez, S.*
Development of an efficient conjugal DNA transfer system between Escherichia coli and a non-sporulating Streptomyces strain.
J. Microbiol. Meth. 144:60-66. Doi:10.1016/j.mimet.2017.11.006. IF: 2.41
Demain, A.L. and Sánchez, S.
Enzymes of industrial interest.
Mexican Journal of Biotechnology 2(2):74-97
|Corina Diana Ceapa||Posdoctoral|
|Dulce María Ramírez Rendón||Doctorado|
|Karol Rodríguez Peña||Doctorado|
|Silvia Margarita Guzmán Trampe †||Doctorado|
|Brenda Gómez Ortiz||Maestría|
|Daniela Stefany Rodríguez Luna||Maestría|
|José Augusto Zelarayan Aguero||Maestría|
|María Paula Gómez Román||Maestría|
|Nidia Maldonado Carmona||Maestría|
|Omar Jiménez Rodríguez||Maestría|
|Sarai Bata Ventura||Maestría|
|Berenice Hernández Cordero||Licenciatura|
|Katherine Paredes Villasana||Licenciatura|