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BIOCHEMICAL AND MOLECULAR BIOLOGY STUDIES ON THE MICROBIAL SYNTHESIS OF SECONDARY METABOLITES.
Secondary metabolites are compounds with a wide range of chemical structures and biological activities synthesized by a few range of microorganisms usually at the late growth phase. Although involved in different functions (signalling differentiation, mediating ecological interactions and facilitating biochemical evolution in microorganisms), these metabolites are considered as secondary since are not essential for microbial growth. The remarkable diversity of biological functions and chemical structures characteristic of these metabolites reflects the random manner in which their biosynthesis has evolved and been exploited. It represents a form of inventive evolution in which metabolic opportunities that arose by chance were exploited and incorporated to various degrees into the phenotype of the species. Therefore it has been interesting for us to elucidate, at the biochemical and molecular biology levels, how these microorganisms are able to synthesize and regulate the production of these compounds. The impact of these studies can be situated at the general microbiology level, but also from the biotechnology standpoint since the resulting knowledge can be used to design rational genetic improvement and process optimization of secondary metabolites production.
PLANT-ASSOCIATED MICROBIAL SYSTEMS AS A SOURCE OF BIOLOGICALLY ACTIVE COMPOUNDS.
Plants and microorganisms synthesize most of the secondary metabolites with biological activity. Almost 50% of the biopharmaceutical have been obtained from plants. Plan-associated microorganisms (endophytic) such as bacteria or fungi produce many of these secondary metabolites. One endophytic fungi is Taxomyces andreanae, able to produce the anticancer drug, Taxol. Other endophytics have been shown to produce compounds with antibacterial, antiviral, antifungal and antimalarial activity. We are interested in the evaluation of medicinal plants as source of endophytic microorganisms as a way to look for new biologically active compounds.
Trenado-Uribe, M., Silva-Miranda, M., Rivero-Cruz, J.F., Rodríguez-Peña, K., Espitia-Pinzón, C., Rodríguez-Sanoja, R., and Sánchez S.
Antimycobacterial activity of an anthracycline produced by an endophyte isolated from Amphipterygium adstringens.
Mol. Biol. Rep. DOI: 10.1007/s11033-018-4424-0. Print ISSN 0301-4851, Online ISSN 1573-4978 IF: 1.889
Armenta, S., Sánchez-Cuapio Z. Munguia ME, Pulido NO, Farrés A, Manoutcharian K, Alejandra Hernandez-Santoyo Silvia Moreno-Mendieta Sergio Sánchez Romina Rodríguez-Sanoja.
The role of conserved non-aromatic residues in the Lactobacillus amylovorus α-amylase CBM26-starch interaction.
Int. J. Biol. Macromol. 2018. 121: 829-838. DOI: 10.1016/j.ijbiomac.2018.10.061. IF: 3.9 https://doi.org/10.1016/j.ijbiomac.2018.10.061
Rodríguez-Peña, K., Macías-Rubalcava, ML., Rocha-Zavaleta, L., Trenado-Uribe, M., Rodríguez-Sanoja, R., and Sanchez S. Streptomyces scabrisporus, an endophyte isolated from Amphipterygium adstringens as producer of an anthracycline active against different cancer cell lines.
Global Drugs Therap. 3(5): 1-10. doi: 10.15761/GDT.1000158 https://www.oatext.com/pdf/GDT-3-158.pdf
Sanchez, S. and Demain, A.L.
Advancement of biotechnology by genetic modifications.
In: Barredo J (Ed) Microbial Carotenoids from Fungi: Methods and Protocols. Humana Press/Springer, New York.
Sanchez S, Ruiz-Villafán B, Ceapa C.D., and Demain A.L.
Synthesis of secondary metabolites and strain improvement current trends and future prospects. In: Fermentation Microbiology and Biotechnology.
4th edition EMT EL-Mansi, CFA Bryce, Dahhou B, Sanchez S, AL Demain, and AR Allman (Eds.). CRC Press; Taylor and Francis Group, Boca Raton. Chapter 4, en prensa
|Corina Diana Ceapa||Posdoctoral|
|Dulce María Ramírez Rendón||Doctorado|
|Karol Rodríguez Peña||Doctorado|
|Silvia Margarita Guzmán Trampe †||Doctorado|
|Brenda Gómez Ortiz||Maestría|
|Daniela Stefany Rodríguez Luna||Maestría|
|José Augusto Zelarayan Aguero||Maestría|
|María Paula Gómez Román||Maestría|
|Omar Jiménez Rodríguez||Maestría|
|Sarai Bata Ventura||Maestría|
|Berenice Hernández Cordero||Licenciatura|
|Diana Enríquez Vargas||Licenciatura|
|Katherine Paredes Villasana||Licenciatura|
|Sebastian de la Rosa Hernández Garibay||Licenciatura|