Áreas de Trabajo
Estudio de mecanismos moleculares que regulan la expresión de glicoproteínas recombinantes con interés terapéutico, producidas en sistemas procariotas o eucariotas, para diseñar formas de cultivo que incrementen su productividad.
Publicaciones Recientes
Muñoz-Echeverri LM, Benavides-López S, Geiger O, Trujillo-Roldán MA, Valdez-Cruz NA
Bacterial extracellular vesicles: biotechnological perspective for enhanced productivity.
World Journal of Microbiology and Biotechnology. 40:174 DOI: 10.1007/s11274-024-03963-7
2024
González-Hernández, R.A., Valdez-Cruz, N.A. & Trujillo-Roldán, M.A.
Factors that influence the extraction methods of terpenes from natural sources.
Chem. Pap. DOI: 10.1007/s11696-024-03339-z
2024
Valdez‑Cruz NA *, Rosiles-Becerril D, Martínez-Olivares CE, García‑Hernández E, Cobos-Marín L, Garzón D, López-Salas FE, Zavala G, Luviano A, Olvera A, Alagón A, Ramírez OT, Trujillo‑Roldán MA *
Oral administration of a recombinant modified RBD antigen of SARS-CoV-2 as a possible immunostimulant for the care of COVID-19.
Microbial cell factories 23, 41 DOI: 10.1186/s12934-024-02320-5
2024
Romero-Martínez, D., Parra-Saldivar, R., Trujillo-Roldán, M.A., Valdez-Cruz, N.A. Bacterial Laccases.
Chapter 7 – Emerging contaminants and their possible bioremediation through bacterial laccases.
Pages 141-172. Bacterial Laccases, Engineering, Immobilization, Heterologous Production, and Industrial Applications. A volume in Progress in Biochemistry and Biotechnology. Book. Elsevier. https://www.sciencedirect.com/book/9780323918893/bacterial-laccases
2023
Valdez-Cruz, N. A., & Trujillo-Roldán, M. A.
Thermoinducible E. coli for Recombinant Protein Production in Inclusion Bodies.
In Inclusion Bodies: Methods and Protocols (pp. 17-30). New York, NY: Springer US
2023
Publicaciones Recientes (continuación)
González-Hernández, R.A., Valdez-Cruz, N.A., Macías-Rubalcava, M.L., Trujillo-Roldán M.A.
Overview of fungal terpene synthases and their regulation.
World J Microbiol Biotechnol 39, 194 DOI: 10.1007/s11274-023-03635-y
2023
Avello V, Torres M, Vergara M, Berrios J, Valdez-Cruz NA, Acevedo C, Sampayo MM, Dickson AJ, Altamirano C.
Enhanced recombinant protein production in CHO cell continuous cultures under growth-inhibiting conditions is associated with an arrested cell cycle in G1/G0 phase.
PLOS ONE 17(11): e0277620. DOI: 10.1371/journal.pone.0277620
2022
Restrepo-Pineda S, Rosiles-Becerril D, Vargas-Castillo AB, Ávila-Barrientos LP, Luviano A, Sánchez-Puig N, García Hernández E, Pérez NO, Trujillo-Roldán MA, Valdez-Cruz NA.
Induction temperature impacts the structure of recombinant HuGM-CSF inclusion bodies in thermoinducible E. coli.
Electronic Journal of Biotechnology. 59: 96-106 DOI: 10.1016/j.ejbt.2022.08.004. [2021 journal impact factor 2.826]
2022
Serrano-Carreón L, Aranda-Ocampo S, Balderas-Ruíz KA, Juárez AM, Leyva E, Trujillo-Roldán MA, Valdez-Cruz NA, Galindo E.
A case study of a profitable mid-tech greenhouse for the sustainable production of tomato, using a biofertilizer and a biofungicide.
Electronic Journal of Biotechnology. DOI: 10.1016/j.ejbt.2022.06.003
2022
Avila-Barrientos LP, Cofas-Vargas LF, Agüero-Chapin G, Hernández-García E, Ruiz-Carmona S, Valdez-Cruz NA, Trujillo-Roldán MA, Weber J, Ruiz-Blanco YB, Barril X, Garcia-Hernandez E.
Computational design of inhibitors targeting the catalytic subunit of Escherichia coli FOF1-ATP synthase.
Antibiotics 11(5):557; DOI: 10.3390/antibiotics11050557 [2020 journal impact factor 4.639]. Online ISSN: 2079-6382
2022
Restrepo-Pineda S, Sánchez-Puig N, Pérez NO, García-Hernández E, Valdez-Cruz NA, Trujillo-Roldán MA*.
The pre-induction temperature affects recombinant HuGM-CSF aggregation in thermoinducible Escherichia coli.
Applied Microbiology and Biotechnology. DOI: 10.1007/s00253-022-11908-z. ISSN: 0175-7598 (print); 1432-0614 (web) [2020 journal impact factor 4.813]
2022
Pérez-Rodriguez S., Reynoso-Cereceda GI, Valdez-Cruz NA, Trujillo-Roldán MA.
A comprehensive comparison of mixing and mass transfer in shake flasks and their relationship with MAb productivity of CHO cells.
Bioprocess and Biosystems Engineering. DOI: 10.1007/s00449-022-02722-y. [2020 journal impact factor 3.210]
2022
Campos-López A, Uribe-López JA, Cázares-Ordoñez V, Garibay-Orijel R, Valdez-Cruz NA, Trujillo-Roldán MA.
Quercetin and 1-methyl-2-oxindole mimic root signaling that promotes spore germination and mycelial growth of Gigaspora margarita. Mycorrhiza.
DOI: 10.1007/s00572-022-01074-5. Electronic ISSN 1432-1890, Print ISSN 0940-6360 [2020 journal impact factor 3.387]
2022
Vargas-Zapata JD, Vargas-Zapata A, Ramírez-Vargas JF, Valdez-Cruz NA, Trujillo-Roldán MA, Orozco-Sánchez F.
Development and characterization of an alternative control system to study separately oxygen and momentum transfer effects in stirred tank bioreactors.
Revista Mexicana de Ingeniería Química. 21(1):Proc2607. DOI: 10.24275/rmiq/Proc2607. ISSN 2395-8472. [2020 journal impact factor 2.148]
2022
Valdez-Cruz NA, Orozco-Sánchez F, Rodríguez-Monroy M, Busto VD, Trujillo-Roldán MA *.
Enseñanza de la operación en biorreactores y el escalamiento de bioprocesos: capacitación multidisciplinaria en un curso teórico-práctico.
Revista Educación en Ingeniería. 17(33), pp. 79-85 https://educacioneningenieria.org/index.php/edi/article/view/1153/1037
2022
Balderas-Ruíz, K. A., Gómez-Guerrero, C. I., Trujillo-Roldán, M. A., Valdez-Cruz, N. A., Aranda-Ocampo, S., Juárez, A. M., … & Serrano-Carreón, L.
Bacillus velezensis 83 increases productivity and quality of tomato (Solanum lycopersicum L.): pre and postharvest assessment.
Current Research in Microbial Sciences, 100076
2021
García-Cabrera, R. I., Valdez-Cruz, N. A., Daniel-Vázquez, A., Blancas-Cabrera, A., & Trujillo-Roldán, M. A.
Roles of culture media and oxygen transfer in the scale-up from shake flasks to pneumatic bioreactor of the plant growth-promoting bacterium Rhizobium phaseoli.
Revista Mexicana de Ingeniería Química, 20(2), 1093-1111
2021
Restrepo-Pineda S, Pérez NO, Valdez-Cruz NA, Trujillo-Roldán MA. Thermoinducible expression system for producing recombinant proteins in Escherichia coli: advances and insights.
FEMS Microbiology Reviews, DOI: 10.1093/femsre/fuab023
2021
Valdez-Cruz NA, García-Hernández E, Espitia C, Cobos-Marín L, Altamirano C, Bando-Campos CG, Cofas-Vargas LF, Coronado-Aceves EW, González-Hernández RA, Hernández-Peralta P, Juárez-López D, Ortega-Portilla PA, Restrepo-Pineda S, Zelada-Cordero P, Trujillo-Roldán MA*.
Integrative overview of antibodies against SARS-CoV-2 and their possible applications in COVID-19 prophylaxis and treatment.
Microbial Cell Factories. 20, 88. DOI: 10.1186/s12934-021-01576-5
2021
Pérez-Rodriguez S, Wulff T, Gunnar Voldbor B, Altamirano C, Trujillo-Roldán MA, Valdez-Cruz NA.
Compartmentalized proteomic profiling outlines the crucial role of the Classical Secretory Pathway during recombinant protein production in Chinese hamster ovary cells.
ACS Omega DOI: 10.1021/acsomega.0c06030
2021
Pérez-Rodriguez S, de Jesús Ramírez-Lira M, Wulff T, Voldbor BG, Ramírez OT, Trujillo-Roldán MA, Valdez-Cruz NA.
Enrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis.
PLoS One. 2020 Aug 25;15(8):e0237930. doi: 10.1371/journal.pone.0237930. eCollection. PMID: 32841274
2020
Pérez-Rodriguez S, Ramírez-Lira MJ, Trujillo-Roldán MA, Valdez-Cruz NA.
Nutrient supplementation strategy improves cell concentration and longevity, monoclonal antibody production and lactate metabolism of Chinese hamster ovary cells.
Bioengineered. 2020 Dec;11(1):463-471. doi: 10.1080/21655979.2020.1744266. PMID: 32223359
2020
Pérez-Rodriguez S, Ramírez OT, Trujillo-Roldán MA, Valdez-Cruz NA.
Comparison of protein precipitation methods for sample preparation prior to proteomic analysis of Chinese hamster ovary cell homogenates.
Electronic Journal of Biotechnology. doi: 10.1016/j.ejbt.2020.09.006
2020
Ángeles-Argáiz RE, Carmona-Reyes IA, Quintero-Corrales CA, Medina-Macías FJ, Blancas-Cabrera A, Valdez-Cruz NA, Ulloa M, Trujillo-Roldán MA, Garibay-Orijel R.
From field sampling to pneumatic bioreactor mycelia production of the ectomycorrhizal mushroom Laccaria trichodermophora.
Fungal Biol. 2020 Mar-Apr;124(3-4):205-218. doi: 10.1016/j.funbio.2020.02.003. Epub 2020, PMID: 32220381
2020
García-Cabrera, R. I., Valdez-Cruz, N. A., Blancas-Cabrera, A., & Trujillo-Roldán, M. A.
Oxygen transfer rate affect polyhydroxybutyrate production and oxidative stress response in submerged cultures of Rhizobium phaseoli.
Biochemical Engineering Journal, 162, 107721
2020
Arregui L, Ayala M, Gómez-Gil X, Gutiérrez-Soto G, Hernández-Luna CE, Herrera de los Santos M, Levin L, Rojo-Domínguez A, Romero-Martínez D, Saparrat MCN, Trujillo-Roldán MA, Valdez-Cruz NA.
Laccases: structure, function, and potential application in water bioremediation.
Microbial Cell Factories 18:200. DOI: 10.1186/s12934-019-1248-0
2019
Restrepo-Pineda S, Bando-Campos CG, Valdez-Cruz NA, Trujillo-Roldán MA*
Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: The role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies.
Cell Stress and Chaperones. IF. 2.571. doi.org/10.1007/s12192-019-01006-x.
2019
Gamboa-Suasnavart RA, Marín-Palacio LD, López-Griego L, Córdova-Aguilar MS, Valdez-Cruz NA, Trujillo-Roldán MA*
Volumetric power input as a reliable parameter for scale-up from shake flask to stirred-tank bioreactor: production of a recombinant glycoprotein by Streptomyces lividans.
Revista Mexicana de Ingeniería Química. 18, (3) 1083-1097. I.F.: 0.924.
2019
Miguel Cuevas-Cruz *, Fernando Lazcano-Pérez, Ulises Hernández-Guzmán, Karen Helena Díaz de la Vega-Castañeda, Sergio A. Román-González, Norma Adriana Valdez-Cruz, Benjamín Velasco-Bejarano, Ana Laura Colín-González, Abel Santamaría, Saul Gomez-Manzo, Jaime Marcial-Quino, Roberto Arreguín-Espinosa *
A novel phospholipase A2 isolated from Palythoa caribaeorum possesses neurotoxic activity
Toxins 11, 89; doi:10.3390/toxins11020089 FI 3.273
2019
Bando-Campos G, Juárez-López D, Román-González SA, Castillo-Rodal AI, Olvera C, López-Vidal Y, Arreguín-Espinosa R, Espitia C, Trujillo-Roldán MA, Valdez-Cruz NA.
Recombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pastoris (Komagataella phaffii) as a tool to study tuberculosis infection.
Microbial Cell Factories. 18:11. Doi: 10.1186/s12934-019-1059-3. I.F. 3.831.
2019
De Marco A, Ferrer-Miralles N, Garcia-Fruitós E, Mitraki A, Peternel S, Rinas U, Trujillo-Roldán MA, Valdez-Cruz NA, Vázquez E, Villaverde A.
Bacterial inclusion bodies are industrially exploitable amyloids. FEMS Microbiology Reviews.
43(1) 53-72, fuy038. doi: 10.1093/femsre/fuy038. I.F. 12.198. Online ISSN 1574-6976 Print ISSN 0168-6445.
2019
Gamboa-Suasnavart RA, Valdez-Cruz NA, Gaytan-Ortega G, Reynoso-Cereceda GI, Cabrera-Santos D, López-Griego L, Klöckner W, Büchs J, Trujillo-Roldán MA.
The metabolic switch can be activated in a recombinant strain of Streptomyces lividans by a low oxygen transfer rate in shake flasks.
Microbial Cell Factories. 17:189. DOI: 10.1186/s12934-018-1035-3. I.F. 3.831.
2018
Vergara M, Torres M, Müller A, Avello V, Acevedo C, Berrios J, Reyes JG, Valdez-Cruz NA, Altamirano C
High glucose and low specific cell growth but not mild hypothermia improve specific r-protein productivity in chemostat culture of CHO cells
PLoS ONE 13(8): e0202098. DOI 10.1371/journal.pone.0202098. FI 3.54
2018
Carlos Calcines-Cruz, Alejandro Olvera, Ricardo M. Castro-Acosta, Guadalupe Zavala, Alejandro Alagón, Mauricio A. Trujillo-Roldán, and Norma A. Valdez-Cruz.
Recombinant-phospholipase A2 production and architecture of inclusion bodies are affected by pH in Escherichia coli.
International Journal of Biological Macromolecules doi:10.1016/j.ijbiomac.2017.10.178
2018
Greta I. Reynoso-Cereceda, María S. Córdova-Aguilar, Norma A. Valdez-Cruz, Mauricio A. Trujillo-Roldán*
Shaken flasks by resonant acoustic mixing in the biosynthesis of alginate by Azotobacter vinelandii with non-Newtonian rheological characteristics.
Journal of Chemical Technology & Biotechnology doi: 10.1002/jctb.5475
2017
Norma A. Valdez-Cruz*, Greta I. Reynoso-Cereceda, Saumel Pérez-Rodríguez, Sara Restrepo-Pineda, Jesús González-Santana, Alejandro Olvera, Guadalupe Zavala, Alejandro Alagón, and Mauricio A. Trujillo-Roldán
Production of a recombinant phospholipase A2 in Escherichia coli using resonant acoustic mixing that improves oxygen transfer in shake flasks.
Microbial cell Factories. 16:129. doi: 10.1186/s12934-017-0746-1
2017
Andrea Bedoya-López, Karel Estrada, Alejandro Sanchez-Flores, Octavio T. Ramírez, Claudia Altamirano, Lorenzo Segovia, Juan Miranda-Ríos, Mauricio A. Trujillo-Roldán, Norma A. Valdez-Cruz.
Effect of temperature downshift on transcriptomic responses of Chinese hamster ovary cells during recombinant tPA production cultures
PLoS One. Online ISSN: 1932-6203. F. 3.234. 11(3):e0151529. doi: 10.1371/journal.pone.0151529
2016
Reynoso-Cereceda GI, Garcia-Cabrera RI, Valdez-Cruz NA, Trujillo-Roldán MA
Shaken flasks by Resonant Acoustic Mixing versus orbital mixing: Mass transfer coefficient kLa characterization and Escherichia coli cultures comparison
Biochemical Engineering Journal. 105: 379-390
2016
Karen Carrasco, Ramsés I. García-Cabrera, Andrea Bedoya-López, Mauricio A. Trujillo-Roldán and Norma A. Valdez-Cruz*
Positive effect of reduced aeration rate on growth and stereospecificty of DL-malic acid consumption by Azospirillum brasilense: improving the shelf life of a liquid inoculant formulation
Journal of Biotechnology 195 (2015) 74–81
2015
Andrea Castellanos-Mendoza, Ricardo M Castro-Acosta, Alejandro Olvera, Guadalupe Zavala, Miguel Mendoza-Vera, Enrique García-Hernández, Alejandro Alagón, Mauricio A Trujillo-Roldán and Norma A Valdez-Cruz
Influence of pH control in the formation of inclusion bodies during production of recombinant sphingomyelinase-D in Escherichia coli
Microbial Cell Factories, 13:137
2014
Luz D. Marín-Palacio, Ramsés A. Gamboa-Suasnavart, Norma A. Valdez-Cruz, Luis Servín-González, Ma. Soledad Córdova-Aguilar, Enrique Soto, Wolf Klöckner, Jochen Büchs and Mauricio A. Trujillo-Roldán
The role of volumetric power input in the growth, morphology, and production of a recombinant glycoprotein by Streptomyces lividans in shake flask cultures
Biochemical Engineering Journal doi: DOI: 10.1016/j.bej.2014.06.010.
2014
Monserrat García-García, Leticia Rocha-Zavaleta, Norma A. Valdez-Cruz, Mauricio A. Trujillo-Roldán
Conservation of the mycelia of the medicinal mushroom Humphreya coffeata
(Berk.) Stey. in sterile distilled water, MethodsX 1:19-22.
2014
Mauricio A. Trujillo-Roldán *, Norma A. Valdez-Cruz, César Gonzalez-Monterrubio, Eduardo V. Acevedo-Sánchez, Carlos Martínez-Salinas, Ramsés I. García-Cabrera, Ramsés A. Gamboa-Suasnavart, Luz D. Marín-Palacio, Jesús Villegas, Abel Blancas-Cabrera
Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation
Applied Microbiology and Biotechnology, 97:9665-9674 . ISSN: 0175-7598. IF. 3.689.
2013
Gamboa-Suasnavart RA, Marín-Palacio LD, Martínez-Sotelo JA, Espitia C, Servín-González L, Valdez-CruzNA, Trujillo-Roldán MA.
Scale-up from shake flasks to bioreactor, based on power input and Streptomyces lividans morphology, for the production of recombinant APA (45/47kDa protein) from Mycobacterium tuberculosis.
World J Microbiol Biotechnol.
2013 Mar 10
Clement H, Costa de Oliveira V, Zamudio FZ, Lago NR,Valdez-CruzNA, Bérnard Valle M, Hajos SE, Alagón A, Possani LD, de Roodt AR.
Isolation, amino acid sequence and biological characterization of an “aspartic-49” phospholipase A2 from Bothrops (Rhinocerophis) ammodytoides venom.
Toxicon. 60(7):1314-23.
2012 Dec 1
Daniel Aguilar-Salvador, Isabel Angeles-Santander, Mauricio A. Trujillo-Roldán, Norma A. Valdez-Cruz*.
Biología Sintética: diseñando sistemas biológicos con piezas genéticas
Revista de Biotecnología. Vol 16, no 1.
2012
Valdez Cruz NA., Romo Buchelly RJ., Álvarez AC., Trujillo-Roldán MA.
Desde el gen hasta el biorreactor en la producción de proteínas recombinantes.
Libro Escalado de bioprocesos y entrenamiento en operación de biorreactores, Capítulo 11, pp. 104-123. ISBN 978-958-761-203-5.
2012
Gamboa-Suasnavart RA, Valdez-Cruz NA, Cordova-Davalos LE, Martínez-Sotelo JA, Servín-Gonzalez L, Espitia C and Trujillo-Roldán MA.
The O-mannosylation and production of recombinant APA (45/47 KDa) protein from Mycobacterium tuberculosis in Streptomyces lividans is affected by culture conditions in shake flasks.
Microbial Cell Factories 2011, 10:110
2011 December 20
Valdez-Cruz NA, Ramírez OT, Trujillo-Roldán MA.
Heat shock molecular responses caused by the production of recombinant proteins in the heat inducible expression system: E. coli with lambda phage pL and/or pR promoters.
Bioengineered Bugs, 2: 2.
2011
Estudiantes
| Nombre | Nivel |
|---|---|
| Giroshi Bando Campos | Doctorado |
| Laura Maria Muñoz Echeverri | Doctorado |
| Santiago Benavides López | Doctorado |
| Daniel Romero Martínez | Maestría |
| Mayra Herrera de los Santos | Maestría |
| Constanza Estefania Martínez Olivares | Estancia |
| Diego Rosiles Becerril | Estancia |